Journal: BioMed Research International
Article Title: A Novel Animal Model of Impaired Glucose Tolerance Induced by the Interaction of Vitamin E Deficiency and 60 Co Radiation
doi: 10.1155/2015/457246
Figure Lengend Snippet: Expression of insulin signaling proteins following different treatments. Western blotting was performed to assess the expression of p-PDK1, PDK1, p-Akt, Akt, p-IRS1, IRS1, and GLUT4. The values from densitometry were normalized to the level of GAPDH, respectively. * P < 0.05 compared to the corresponding experimental control group, # P < 0.05 compared to the corresponding VE deficiency group, and Δ P < 0.05 compared to the corresponding radiation group.
Article Snippet: Primary antibodies against phosphor-insulin receptor substrate 1 (p-IRS1, Cat. number 2386), IRS1 (Cat. number 3407), phosphor-phosphoinositide-dependent protein kinase 1 (p-PDK1, Cat. number 3438), PDK1 (Cat. number 5662), phosphor-amino kinase terminal (p-Akt, Cat. number 4060) and Akt (Cat. number 4691) were obtained from Cell Signaling Technology.
Techniques: Expressing, Western Blot